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Human brain cut into 7,400 slices, then reconstructed digitally in 3-D

Montreal Neurological Institute / McGill

The "BigBrain" 3-D model will give researchers a high-resolution look at the human brain.

LONDON — Scientists have finely sliced a human brain into 7,400 wafer-thin sheets and then digitally reconstructed it to create the world's most detailed map of the brain in three dimensions.

The "BigBrain" project, which took a 65-year-old woman's brain and cut it into slices just 20 micrometers thick, shows the brain's anatomy in microscopic detail, almost down to a cellular level, said the scientists who carried out the work. A micrometer, or micron, is a millionth of a meter, or less than a ten-thousandth of an inch.

The resulting 3-D reference brain will allow researchers worldwide to study the organ in unprecedented physical detail, paving the way for deeper understanding of how processes like cognition and emotions work, and how brain diseases develop.


'Big science'
"This is big science comes to the brain," said Alan Evans, a professor at the Montreal Neurological Institute at McGill University in Montreal, Canada, who co-led the study. "We've raised the level of insight (by) orders of magnitude. ... This data will revolutionize our ability to understand internal brain organization."

Neuroscientists have created reference brains in the past and used them to study how physical form relates to the brain's chemical signaling and genetic makeup, but this is the first 3-D brain map to offer such detail — at a spatial resolution of 20 microns, smaller than the size of one fine strand of hair.

Until now, scientists had only been able to study the brain on a scale of about 1 millimeter, Evans told reporters in a teleconference. "We're now 50 times smaller in all three dimensions," he said. "This changes the game in terms of our ability to discriminate very fine structural and physiological properties of the human brain."

Amunts et al. via AP

Researchers cut sections from a brain preserved in paraffin wax into slivers that measure just 20 micrometers thick, resulting in more than 7,400 slices.

How the brain was sliced
Because the sliced and reconstructed BigBrain was created from a dead organ, scientists said the best way to imagine it was as a type of scaffold providing a 3-D framework for living brain information to be analyzed.

Using a special tool called a microtome, the researchers from Canada and Germany sliced the 65-year-old human female brain, which had been embedded in paraffin wax, into more than 7,400 sections. They then mounted the 20-micron-thick sections onto slides, stained them to detect cell structures and then digitized them with a high-resolution scanner so that the 3-D brain model could be reconstructed. It took around 1,000 hours to collect the data.

The fine-grained anatomical resolution should give scientists insights into the neurobiological basis of cognition, language, emotions and other brain processes.

The researchers "pushed the limits of current technology," said Peter Stern, editor of the journal Science, where the team's work was published this week.

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